本发明提供人工构建几丁质小体多酶复合体scaford‑chiC‑chiA‑sg的方法及应用。多酶复合体利用来源于自然界中的纤维小体中存在的相互作用蛋白对，人工融合表达其中的粘连蛋白(cohesins)构建蛋白支架，并在相应的几丁质内切酶、几丁质外切酶、N‑乙酰氨基葡萄糖苷酶上融合表达锚定蛋白，从而使其胞外自组装得到几丁质酶多酶复合体；然后将多酶复合体应用于几丁质的降解制备N‑乙酰氨基葡萄糖。本发明多酶复合体不但实现了游离几丁质酶的胞外自组装，而且固定了酶与酶之间的距离，提升体系中酶元件的协同效应，形成底物通道，从而提高了对几丁质的降解效率，得到单一的单糖产物，降低了生产和后续分离的成本。

The present invention provides a method and application for artificially constructing chitin body multi enzyme complex scaford chiC chiA sg. The multi enzyme complex utilizes the interacting protein pairs present in natural fibrous bodies to artificially fuse and express the adhesive proteins (cohesins) to construct a protein scaffold. The anchor protein is fused and expressed on the corresponding chitin endonuclease, chitin exonuclease, and N-acetylglucosamine glycosidase, resulting in its extracellular self-assembly to obtain the chitinase multi enzyme complex; Then, the multi enzyme complex is applied to the degradation of chitin to prepare N-acetylglucosamine. The multi enzyme complex of the present invention not only achieves extracellular self-assembly of free chitinase, but also fixes the distance between enzymes, enhances the synergistic effect of enzyme elements in the system, forms substrate channels, thereby improving the degradation efficiency of chitin, obtaining a single monosaccharide product, and reducing production and subsequent separation costs.