为进一步评价P-PUU支架的骨修复效果和体内生物相容性，采用大鼠胫骨修复模型进行为期8周的植入试验。microCT图像（图5）表明，与未经处理的缺陷（空白组）相比，植入PDLLA和P-PUU支架可以明显促进新骨的形成缺陷地区和新骨P-PUU支架的数量大大大于PDLLA支架。此外，随着PP含量的增加，P-puu中的新骨量逐渐增加。有趣的是，在植入的支架上沿着细丝观察到少量的新骨形成，这表明三维多孔支架作为模板引导新骨生长到相互连接的孔中，特别是对于P-PUU支架。从micro-CT图像中定量分析新形成的骨(图5B)，包括骨体积密度(骨体积/组织体积、BV/TV)、骨小梁厚度(Tb。、小梁数（Tb.N）和小梁分离（Tb。Sp)，表示BV/TV、Tb。随着PP含量的增加，含量下降，表明3d打印多孔支架具有良好的成骨能力和PP的掺入可进一步提高其成骨能力。

To further evaluate the bone repair efficacy and in vivo biocompatibility of the P-PUU scaffolds, a rat tibia repair model was used for implantation test of 8 weeks. The microCT images (Figure 5A) indicate that, compared with the untreated defects (blank groups), implantation of both PDLLA and P-PUU scaffolds could obviously promote formation of new bone in defected regions and the amount of new bone in P-PUU scaffolds was substantially greater than that in PDLLA scaffolds. Moreover, with the increase of PP contents, the amount of new bone in P-PUUs was gradually improved. Interestingly, a small amount of new bone was observed to form along the filaments of the implanted scaffolds, suggesting that the 3D porous scaffolds as a template guiding new bone ingrowth to the interconnected pores, especially for P-PUU scaffolds. Quantification of the newly formed bone from the micro-CT images (Figure 5B), including the bone volume density (bone volume/tissue volume, BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp), indicated that BV/TV, Tb.Th, and Tb.N improved yet Tb.N declined with the increase of PP contents, demonstrating that 3D-printed porous scaffolds have an excellent osteogenic capability and incorporation of PP could further enhance their bone formation ability.