图A直接显示了DAPI染色活细胞培养7天后MC3T3-E1在不同支架上的细胞分布。进一步收集罗丹明-磷青素和DAPI染色的高倍图像(图B),揭示了各种支架大孔中的细胞形态和生长情况。可以看出,所有的3d打印聚合物支架都能支持细胞生长,并通过相互连接良好的大孔进一步刺激细胞进入支架。然而,P-PUUs支架上的细胞分布和活力均优于PDLLA支架。此外,通过增加PP,可以改善P-PUU支架上细胞的增殖(图C)。其中,P-PUU1.4支架上的细胞增殖能力明显优于PDLLA支架。同样,随着PLP活性的增加,成骨细胞分化随PP含量的增加而增加,P-PUU1.4支架在每个时间点促进ALP活性显著高于PDLLA。
A directly shows the cell distribution of MC3T3-E1 on various scaffolds after culture of 7 days by staining the live cells as blue with DAPI. The highmagnifification images of rhodamine-phalloidin and DAPI staining were further collected (B) to reveal the cell morphologies and ingrowth in the macropores of various scaffolds. It can be seen that all the 3D-printed polymeric scaffolds could support cells growth and further stimulate cells ingrowth to scaffolds via the well-interconnected macropores. Despite, the distribution and viability of cells on P-PUUs scaffolds were better than those on PDLLA scaffolds. Moreover, the proliferation of cells on P-PUU scaffolds (C) could be improved by increasing PP.Particularly, the proliferation of cells on P-PUU1.4 scaffolds was meaningfully better than those on PDLLA scaffolds. Similarly, the osteoblasts differentiation by ALP activity (D) increased with the increase of PP contents, and P-PUU1.4 scaffolds promoted signifificantly higher ALP activity than PDLLA at each time point.