BSA 的吸收峰在 280 nm 附近被检测到。 新鲜溶液用作对照。 放置一天后未浸泡样品的溶液显示出与新鲜溶液相似的光谱(图 17a),不包括溶液中 BSA 本身的变化。 在前 2 天(图 17b 和 c),峰值强度或位置没有观察到明显变化。 浸泡时间越长,吸收峰强度逐渐增大,最大波长随之移动,表明BSA结构无序。 结合图 10a,浸泡 5-14 天期间 Zn2+ 的释放量较高,这可能是 5 天、8 天和 14 天紫外-可见光谱发生明显变化的原因(图 17d-f) )。
The absorption peak for BSA is detected around 280 nm. The fresh solution is used as control. The solution placed after one day without soaking the sample shows the similar spectrum as the fresh solution (Fig.17a), which excludes the changes of BSA itself in the solution. In the first 2 days (Fig. 17b and c),no obvious change is observed in the peak intensity or position. After longer immersion, the absorption peak intensity is gradually increased,which is followed by the shift in the maximum wavelength, indicating the disorder of the BSA structure. Combined with Fig. 10 a, the released amount of Zn2+ is higher during the immersion between 5–14 days,which may account for the obvious change of UV–vis spectra for 5 d, 8 d and 14 d (Fig. 17d–f).