The invention relates to a TiN/Ag nano-multilayer film with the surface being implanted by silver ions as well as a preparation method and application thereof. The preparation method of the TiN/Ag nano-multilayer film with the surface being implanted by silver ions comprises the following steps: depositing TiN and Ag layers alternately on a single-side polished titanium alloy (Ti6Al4V) substrate, wherein the modulation ratio of TiN to Ag is 6:1, ten modulation periods are set, and the modulation thickness is 50-70 nm; depositing a TiN layer with the thickness of 225-275 nm on the surface of the multilayer film by sputtering to serve as the top layer of the multilayer film to enable the total thickness of the multilayer film to be 1-1.5 [mu]m; and finally, implanting 1.0*10[17]-1.1*10[17] ions/cm[2] of Ag ions in the surface layer of the multilayer film. According to the TiN/Ag nano-multilayer film with the surface being implanted by silver ions as well as the preparation method and application thereof, the hardness of the prepared TiN/Ag nano-multilayer film reaches 32.17 GPa, the TiN/Ag film having a multilayered structure and with the best performance is obtained after testing the mechanical property and biological property of the multilayer film, excellent overall characteristics such as high hardness, high film-substrate cohesion, good hydrophily, biocompatibility and antibacterial property are achieved, and the TiN/Ag nano-multilayer film with the surface being implanted by silver ions and with good biocompatibility will have an important application prospect in the technical fields of biomedical materials and bioengineering films.
