本发明公开一种针对透明质酸酶分泌型细菌的细菌响应性钛基抗菌植入材料制备方法。该方法首先通过阳极氧化法在钛材表面构建二氧化钛纳米管，并将其用做构建抗菌植入材料的载药基底。其次，利用层层自组装技术(旋涂法)构建壳聚糖/透明质酸 天蚕肽B多层膜，从而在载药纳米管基材表面构建出一种细菌(透明质酸酶分泌型细菌)响应性的抗菌生物涂层，以提高钛基移植体的抗菌性能。

The present invention discloses a preparation method of a bacterial responsive titanium-based antibacterial implantation material for hyaluronidase secreting type bacteria. According to the method, titanium dioxide nano-tubes are constructed on the surface of a titanium material through an anode oxidation method, and are adopted as a drug loading substrate for constructing an antibacterial implantation material; and a layer-by-layer self-assembly technology (spin coating method) is used to construct a chitosan/hyaluronic acid-cecropin B multilayer film so as to construct a bacterial (hyaluronidase secreting type bacteria) responsive antibacterial biological coating on the drug loading nano-tube substrate surface, such that the antibacterial property of the titanium-based transplant is improved.