一种基于透明质酸的线粒体靶向抗氧化剂Mito Q纳米制剂的制备方法及应用,本发明利用Mito Q带正电的特点,将他与带负电的透明质酸混合,探索通过高温辅助电荷驱动自组装的方法制备Mito Q/HA抗氧化物纳米粒子,利用纳米粒子包载的方法,提高药物跨膜转运效率,提高Mito Q线粒体靶向效果。

Novel mitochondria-specific anti-oxidative nanoparticles (NPs) consisting of negatively charged hyaluronic acid and the positively charged MitoQ were prepared by charge-driven self-assembly strategy assisted by heat treatment. The effects of operation temperature on size, morphology, zeta potential, and polydispersity index (PDI) of the MitoQ NPs were investigated. The NPs prepared by this strategy exhibited relatively higher drug encapsulation capacity and loading efficiency compared to that prepared from amphiphilic block copolymer using the traditional self-assembly method. The heat treatment enhanced the interaction between the charged molecules HA and MitoQ, thus the resultant NPs had more compact structure with uniform spherical morphology. In comparison with free MitoQ, MitoQ NPs had increased mitochondrial distribution, thereby exhibited a stronger mitochondrial ROS scavenging activity in the tested cell line.