本发明公开了一种胶原基纤维素纳米晶体复合膜的制备方法，包括，分别制备得到胶原溶液和纤维素纳米晶体的水分散液，然后将两者的混合溶液在室温下除去气泡，浇注在培养皿中，风干后得到胶原基纤维素纳米晶体复合膜。本发明制备了具有较好的力学性能和良好的生物相容性的胶原/纤维素纳米晶体复合膜，结合了胶原与纤维素纳米晶体各自的一些优势，制备工艺简单、安全，成本较低，可用于角膜修复的替代材料，使胶原能适应更广泛的生物医学应用。

The invention discloses a preparation method of a collagen-based cellulose nanocrystal composite membrane, and the method comprises the following steps: respectively preparing a collagen solution anda cellulose nanocrystal aqueous dispersion, removing bubbles from a mixed solution of the collagen solution and the cellulose nanocrystal aqueous dispersion at room temperature, pouring the mixed solution into a culture dish, and air drying to obtain the collagen-based cellulose nanocrystal composite membrane. According to the invention, the collagen/cellulose nanocrystal composite membrane with good mechanical properties and good biocompatibility is prepared, the respective advantages of collagen and cellulose nanocrystals are combined, the preparation process is simple, safe and low in cost,and the collagen/cellulose nanocrystal composite membrane can be used as a substitute material for corneal repair, so that the collagen can be widely used in biomedicine.