本发明属于生物医用材料领域，公开了一种耐缝合的胶原基角膜再生修复材料及其制备方法。将胶原加入到盐酸中溶解，然后加入EDC/NHS交联剂进行交联反应，加热浓缩，得到交联胶原溶液，经脱泡后加入到培养皿中至一定厚度，然后将PCL圆环平铺到交联胶原溶液底部，风干，清洗，得到复合膜；将所得复合膜在模具中经100～120℃热压二次成型，得到耐缝合的胶原基角膜再生修复材料。本发明所得耐缝合的胶原基角膜再生修复材料生物相容性良好，耐手术中的常规缝合，并且角膜上皮细胞可以在本材料的表面上粘附和增殖，从而实现手术中角膜上皮细胞在植片上的快速上皮化，最终达到角膜缺损修复和角膜替代的目的。

The invention belongs to the field of biomedical materials, and discloses a suture-resistant collagen-based corneal regeneration and repair material and a preparation method thereof. The preparation method comprises the following steps: adding collagen to hydrochloric acid to dissolve collagen, then adding an EDC/NHS cross-linking agent for a cross-linking reaction, heating and concentrating to obtain a cross-linked collagen solution; defoaming, adding the defoamed product to a petri dish to reach a certain thickness, then tiling a PCL circular ring to the bottom of the cross-linked collagen solution, air-drying, and washing to obtain a composite film; and hot-pressing the composite film in a die at 100-120 DEG C for secondary molding to obtain the suture-resistant collagen-based corneal regeneration and repair material. The suture-resistant collagen-based corneal regeneration and repair material obtained by the invention has good biocompatibility and is resistant to conventional suture during surgery. In addition, corneal epithelial cells can adhere to and proliferate on the surface of the material. Thereby, rapid epithelization of the corneal epithelial cells on the graft duringsurgery is realized, and finally the purpose of corneal defect repair and corneal replacement is achieved.