本发明属于生物医用材料领域，公开了一种具有3D微图案的胶原薄膜及其制备方法和应用。采用具有3D微图案的掩膜版通过光刻技术在硅片上形成3D微图案，然后在硅片上浇注PDMS预聚物，交联固化后得到带有3D微图案的PMDS基底；将胶原溶解于酸性溶液中，加水搅拌均匀，得到胶原溶液，然后加入交联剂交联；将交联反应产物经除泡后浇注在PDMS基底上，风干成膜后剥离，得到具有3D微图案的胶原薄膜。本发明的制备工艺简单，成本较低，所制备的薄膜结合了胶原蛋白和表面图案化的优势，具有良好的理化性能和生物相容性，可用于角膜组织工程，诱导角膜组织再生，并对角膜细胞的行为进行调控，形成与天然组织相似的结构。

The invention belongs to the field of biomedical materials, and discloses a collagen film with 3D micro-patterns as well as a preparation method and application thereof. The collagen film with the 3Dmicro-patterns is prepared by the following steps: forming 3D micro-patterns on a silicon wafer by adopting a photolithography technology by using a mask with 3D micro-patterns; pouring PDMS prepolymers onto the silicon wafer so as to be subjected to cross-linking and curing, thereby obtaining a PDMS base with the 3D micro-patterns; dissolving collagen in an acidic solution, adding water, and carrying out uniform stirring so as to obtain a collagen solution; adding a cross-linking agent into the collagen solution so as to be subjected to cross-linking, thereby obtaining cross-linking reactionproducts; and then, removing bubbles from the cross-linking reaction products, pouring the bubble-removed cross-linking reaction products onto the PDMS base, carrying out air-drying so as to have a film formed, and carrying out stripping so as to obtain the collagen film with the 3D micro-patterns. The preparation technology of the collagen film with the 3D micro-patterns is simple in processes and relatively low in cost; and the prepared film combines advantages of collagen and patterned surface, thereby having good physicochemical properties and biocompatibility. The collagen film with the 3D micro-patterns can be used in corneal tissue engineering so as to induce corneal tissue regeneration; moreover, the collagen film with the 3D micro-patterns is capable of performing regulation on corneal cell behavior, thereby forming a structure similar to native tissues.