将物理凝胶和化学凝胶相结合，利用化学改性明胶水体系的非牛顿流变性能，实现了双层水凝胶多孔支架的连续3D打印。结果表明，具有适当间距的双层水凝胶支架能够较好地在体内再生关节软骨。

A continuous 3D printing of bilayered hydrogel porous scaffolds was achieved by combining both physical gelation and chemical gelation and employing the non-Newtonian rheological properties of the aqueous system of the chemically modified gelatin. The cell-free bilayered hydrogel scaffolds with the appropriate inter-filament spacing were found to well regenerate articular cartilage in vivo.