为了观察RGCs对DFO-NPs的细胞摄取,应用近红外荧光探针(Cy5.5)标记DFO-NPs (Cy5.5-DFO-NPs)。通过荧光显微镜和流式分析仪比较了RGCs对Cy5.5-DFO-NPs和游离Cy5.5的摄取。
In order to observe the cellular uptake of DFO-NPs by RGCs, DFO-NPs (Cy5.5-DFO-NPs) were labeled with near-infrared fluorescent probes (Cy5.5). The uptake of Cy5.5-DFO-NPs and free Cy5.5 by RGCs was compared by fluorescence microscope and flow analyzer.