C11-BODIPY 581/591是一种结合于脂质的膜探针,广泛应用于铁死亡研究中,评价细胞膜(也包括细胞器膜)上脂质ROS水平。用C11-BODIPY 581/591荧光染料染色RGCs后,用流式细胞仪检测ONC组和假手术组的RGCs脂质氧化水平。
C11-BODIPY 581/591 is a lipid-bound membrane probe, which is widely used in iron death studies to evaluate lipid ROS levels on cell membranes (including organelle membranes). After staining RGCs with C11-BODIPY 581/591 fluorescent dye, flow cytometry was used to detect the lipid oxidation level of RGCs in the ONC group and the sham operation group.