先将装微管的试管称重,然后将不同Gel-Ma浓度的微管放在管中并加入1 mL PBS,放入摇床让其溶胀(37 ℃,100 r),按照设定的时间点将微管内的PBS移除,迅速用定性滤纸将微管表面的水分吸干,然后通过电子天平称微管和试管的总重量,减去试管的重量就得到微管的重量,与初始重量相减,得到吸水百分比。用PBS配制不同浓度的GIMs悬液,改变微球在其中的浓度,然后用此悬液配200 mg/mL的Gel-Ma溶液制备得到不同微球浓度的Gel-Ma微管,按照相同的方法,探索不同微球浓度的Gel-Ma管溶胀规律。
First weigh the test tube containing the microtubes, then put the microtubes with different Gel-Ma concentrations in the tube and add 1 mL PBS, put it in a shaker to swell (37 ℃, 100 r), according to the set time Click to remove the PBS in the microtube, quickly absorb the water on the surface of the microtube with a qualitative filter paper, and then weigh the total weight of the microtube and the test tube with an electronic balance, subtract the weight of the test tube to get the weight of the microtube, and the initial weight Subtract to get the percentage of water absorption. Prepare different concentrations of GIMs suspension with PBS, change the concentration of microspheres in it, and then use this suspension to mix 200 mg/mL Gel-Ma solution to prepare Gel-Ma microtubes with different microsphere concentrations, follow the same method , Explore the swelling law of Gel-Ma tubes with different microsphere concentrations.