细胞以1×105细胞/孔的初始播种密度接种于放置在6孔培养板中的样品上,并培养至7天和14天。RNA分离和PCR严格按照检测试剂盒供应商的说明进行。
The cells were seeded in 6-well plates at an initial seeding density of 1×105 cells/well and cultured for 7 and 14 days. RNA separation and PCR were performed in strict accordance with the kit vendor's instructions.