将rBMSCs按细胞密度2×104细胞/毫升接种于材料上。孵育21天后，用2.5%戊二醛固定细胞15min（超纯水配置），用超纯水清洗3次后加入1%的茜素红染色液（超纯水配置，PH=4.2）。37℃染色30min，倒置显微镜拍照。

RBMSCs were inoculated on the material with a cell density of 2×104 cells/ml. After 21 days of incubation, the cells were fixed with 2.5% glutaraldehyde for 15min (ultra-pure water configuration), washed with ultra-pure water for 3 times, and then added with 1% alizarin red staining solution (ultra-pure water configuration, PH=4.2). Staining at 37℃ for 30min, inverted microscope was used to take pictures.