检测到中文(简体) 英语
通用领域
生物医药
本发明公开一种基于液相色谱评价体外辅助生殖用液质量的方法，其包括利用第一色谱柱在高温条件下进行第一检测的步骤，和利用第一色谱柱在低温条件下进行第二检测的步骤；其中，高温是指70-80℃的温度，低温是指25-35℃温度，第一检测和所述第二检测包括使用相同的流动相及流速、相同的检测器。通过本发明方法能够对体外辅助生用液有关成分实现有效的定量和分析，实现对产品质量有效的控制。不仅大大降低分析难度，使各企业具备检测能力，提高体外辅助生殖培养液的质量。而且解决了液相质谱联用法可能导致的较大的误差，而是实现在一次检测过程中对多种目的物质的联合检测。
The invention discloses a method for evaluating the quality of liquid for in vitro assisted reproduction based on liquid chromatography, which comprises a step of performing a first detection under high temperature conditions using a first chromatographic column and a step of performing a second detection under low temperature conditions using a first chromatographic column; Wherein, high temperature refers to the temperature of 70-80 ℃, and low temperature refers to the temperature of 25-35 ℃. The first detection and the second detection include using the same mobile phase, flow rate and the same detector. The method of the invention can realize effective quantification and analysis of relevant components of in vitro auxiliary biological solution, and realize effective control of product quality. It not only greatly reduces the difficulty of analysis, enables enterprises to have the detection ability, and improves the quality of in vitro assisted reproductive culture medium. Moreover, it solves the large error that may be caused by liquid phase mass spectrometry, but realizes the joint detection of multiple target substances in one detection process.
