本发明公开了多重靶向修饰的载基因复合物及制备方法及应用，其方法为：制备膜靶向肽修饰的聚阳离子基因载体(I)；将TAT‑NLS多肽水溶液与核酸水溶液混匀；得混合液；将基因载体(I)配成悬浮液；(4)悬浮液和混合液混匀，静置，得到多重靶向修饰的载基因复合物；本发明的多重靶向修饰的载基因复合物。通过膜靶向肽中的REDV肽与内皮细胞表面的整合素受体特异性识别，提高细胞对其摄取。进入细胞后的复合物位于溶酶体/内涵体结构中，通过聚乙烯亚胺与TAT共同作用，提高该复合物的内涵体逃逸能力，促进目的基因进入细胞质中。通过核定位信号NLS与核膜的相互作用，促进目的基因的核内在化。目的基因在内皮细胞中的转染效率强。

The invention discloses a multi-targeted modified gene-loaded compound, a preparation method and application. The method comprises the following steps: preparing a membrane targeted peptide modified polycation gene vector (I); evenly mixing a TAT-NLS polypeptide aqueous solution and a nucleic acid aqueous solution to obtain mixed liquor, and preparing the gene vector (I) into suspension liquid; and (4) evenly mixing the suspension liquid and the mixed liquor, and standing to obtain the multi-targeted modified gene-loaded compound. The multi-targeted modified gene-loaded compound is specifically recognized by REDV peptide in membrane targeted peptide and an integrin receptor on the surface of an endothelial cell, and uptake of the cell to the compound is improved. The compound which enters the cell is positioned in a lysosome/endosome structure, polyethyleneimine and TAT jointly act, the escaping capacity of the endosome of the compound is improved, and a target gene is promoted to enter cytoplasm. Through interaction of a nuclear localization signal NLS and a nuclear membrane, nuclear internalization of the target gene is promoted. Transfection efficiency of the target gene in the endothelial cells is high.