本发明属于药物制剂领域,具体涉及一种具有药物梯度分布的载药微管及其制备方法。本发明提供一种具有药物梯度分布的载药微管,当其所负载药物为神经生长因子时,可以人为地在人造神经修复导管中提供营养因子的梯度分布,可为轴突提供具有神经生长因子浓度梯度分布的导管,诱导轴突沿着药物浓度梯度实现定向生长。本发明也可以作为其它药物的具有药物梯度分布的载药微管,实现靶向精准释药或者其它给药目的。

A hydrogel microcolumn with gradient drug distributed was prepared by magnetic inducing. magnetic nanoparticles (MNP) were prepared using thermal co-precipitation method, and was further modified with PEG-PGA (PEG-MNP) to achieve a better dispensability and stability in aqueous solution. Then, gelatin solutions containing magnetic nanoparticles were crosslinked in a water-in-oil (W/O) solvent system to prepare PGE-MNP loaded gelatin microspheres (GMP@PEG-MNP). CNTF was loaded into the GMP@PEG-MNP by free diffusion forming a CNTF and PEG-MNP co-loaded gelatin microspheres (CNTF-GMP@PEG-MNP). The CNTF-GMP@PEG-MNP and methacrylate gelatin (Gel-Ma) solutions were mixed in a quartz tube, and were then treated with a magnet to achieving a gradient CNTF distribution in this mixture. The microcolumns with gradient CNTF distribution was obtained after crosslinking of Gel-Ma with UV treatment under the presence of the photoinitiator.